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This example invites the design of C-two-substituted analogs of adenosine nucleotides and NAD+ as selective inhibitors of LigA. In fact, it was described lately that two-methylthio-ATP can be a potent inhibitor of your adenylylation response of E. coli
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) so that the concave area of the OB β-barrel suits in the DNA minor groove. This changeover elicits a 63-Å motion of the OB area and places the latch deep in the DNA main groove.
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The zinc-finger module plays a structural job in bridging the OB and HhH domains. Domain Ia will make no contacts Together with the DNA duplex, in line with its dispensability for catalysis of strand closure on an AppDNA substrate. (No electron density was noticed with the C-terminal BRCT domain.)